Product Description

Advanced BioMatrix offers PhotoAlginate®, a methacrylated alginate for photocrosslinkable hydrogels or for mixing with other photocrosslinkable hydrogels (such as PhotoGel, PhotoCol or PhotoHA). PhotoAlginate® can form 3D gels and can be prepared at various concentrations and photocrosslinked to provide various gel stiffness. PhotoAlginate® is unique in that it can also be crosslinked with calcium. This product can be ordered by itself (200 mg of lyophilized sterile powder), or as a kit that comes with a photoinitiator for photocrosslinking (see table below):

Item Catalog Number Photoinitiator Included
PhotoAlginate #5310 N/A
PhotoAlginate with Irgacure #5353 Irgacure (365nm)
PhotoAlginate with LAP #5354 LAP (405nm)
PhotoAginate with Ruthenium #5355 Ruthenium (400-450nm)

 

 

Once solubilized, the PhotoAlginate® can be stored at 2-10°C for 1 month.

Parameter, Testing, and Method Methacrylated Alginate #5310
Sterilization Method Filtration
Sterility - USP modified No growth
Form Lyophilized Powder
Package Size 200 mg
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Shelf Life After Reconstitution 1 Month
Degree of Methacrylation > 20%
Molecular Weight ~ 30 kDa

 

Directions for Use

3D Hydrogel Preparation:

Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation.

Typical working concentrations are between 10-20 mg/ml (1-2%).

Note: The following instructions are for a 1% hydrogel. 

  1. Add 20 ml of 1X cold phosphate buffer saline (PBS) or cell culture media to the 200 mg of lyophilized methacrylated alginate powder. Ensure buffer does NOT contain calcium, as this would begin crosslinking the alginate. 
  2. Mix on a shaker table or rotator plate until fully solubilized (~60 minutes) at 2-10°C.Note: Solubilization times may vary depending on the desired concentration and volume of PBS or medium added.
  3. Solubilize the photoinitiator at the recommended concentration, and add desired volume to your PhotoAlginate®. See table below for concentration and volume recommendations. 
  4. Add your cells to the solution if desired. Cells can also be added on top of crosslinked hydrogels. For cellular attachment within the hydrogel, we recommend adding another ECM to the mixture (such as collagen). 
  5. Dispense your PhotoAlginate/photoinitiator/cell solution into the desired cultureware (i.e. 6-well plate, 48-well plate, etc...).
  6. Photocrosslink until a hydrogel of the desired stiffness has formed.
  7. Note:  Longer exposure allows more crosslinking, though each cell type withstands different degrees of UV light and free radical exposure (generated by the photoinitiator) that mediates crosslinking.

When 3D Bioprinting with PhotoAlginate®, It is recommended to print using the FRESH method, where the support bath contains a low dose of calcium to immediately crosslink the bioprinted structure prior to photocrosslinking. 

Photoinitiator Photoinitiator Solubilization  Amount to add to PhotoDextran Solution
Irgacure 100 mg/ml in Methanol Multiply total volume of PhotoDextran by 0.01. Add this amount of Irgacure solution. 
LAP 17 mg/ml in 1X PBS or Media Multiply total volume of PhotoDextran by 0.02. Add this amount of LAP solution.

Ruthenium

Sodium Persulfate

Ruthenium - 37.4 mg/ml in 1X PBS or Media

Sodium Persulfate - 119 mg/ml in 1X PBS or Media

 Multiply total volume of PhotoDextran by 0.02. Add this amount of both Ruthenium AND Sodium Persulfate solutions.

Product Certificate of Analysis

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Safety and Documentation

Certificate of Origin

SDS

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.